Marijuana inspection: microbial pathogens and total yeast and mold

 
Concerning the testing of cannabis product for microbiological contaminants,
there is a large pool of standard methods on which to draw. All microbiological methods
employed must include applicable controls. Qualitative pathogen methods must confirm
presumptive results as either positive or negative by the inclusion of a
confirmation step. Confirmation of pathogens should not be addressed by simply
re-running positive sample enrichments or retesting remaining sample.
 
 
Methods applicable to Salmonella spp. and Shiga toxin-producing Escherichia coli testing:
 
Methods applicable to total yeast and mold testing:

 

Methods applicable to total aerobic microbial counts:
 
 
Methods applicable to Staphylococcus aureus testing:
 
 
Methods applicable to Pseudomonas aeruginosa testing:
 
 
Methods applicable to bile tolerant gram negative bacteria:
Note: there are many applicable methods not listed in this section due to the fact that these methods are labeled as “total Enterobacteriacea” which, if modified appropriately, might be applicable for use analyzing total gram negatives, but any method that specifically counts only members of Enterobacteriacea is not applicable.
 
 
Methods applicable to Candida albicans testing:
 
  • Baird, Rosamund M., Corry, Janet E. L., Curtis, Gordon D. W.  2012. Handbook of Culture Media for Food and Water Microbiology 3rd Edition. The Royal Society of Chemistry.
  • Odds, Frank C. and Bernaerts Ria.. 1996. CHROMagar Candida, a New Differential Isolation Medium for Presumptive Identification of Clinically Important Candida Species. Journal of Clinical Microbiology, 32(8) 1923-1929
  • International Standards Organization (ISO), 2015. “Cosmetics -- Microbiology -- Detection of Candida albicans ISO/TS 18416:2015.” https://www.iso.org/standard/25571.html
  • Leber, Amy L. 2016. Clinical Microbiology Procedures Handbook, 4th Edition. American Society for Microbiology.
  • Pfaller M. A., Houston, A., Coffmann. 1996. Application of CHROMagar Candida for Rapid Screening of Clinical Specimens for Candida albicans, Candida tropicalis, Candida krusei, and candida (Torulopsis) glabrata. Journal of Clinical Microbiology, 34(1) 58-61
  • United States Pharmacopeia (USP), 2009. “<61>  Microbiological Examination of nonsterile products: microbial enumeration tests.” https://www.uspnf.com/official-text/accelerated-revision-process/accelerated-revision-history/harmonized-microbiology-general-chapters
United States Pharmacopeia (USP), 2009. “<62>  Microbiological examination of nonsterile products: tests for specified microorganisms.” https://www.uspnf.com/official-text/accelerated-revision-process/accelerated-revision-history/harmonized-microbiology-general-chapters